Maintaining an ideal cell metabolism is critical for optimizing bioprocess quality and yield. Inline monitoring of viable cell density, nutrients, and metabolites is a proven approach to achieving higher yields and consistent product quality. Inline analysis provides an around-the-clock picture of the bioprocess without needing to collect samples for laboratory measurements. This increased knowledge supports advanced process control and reduces the amount of time for process development.

Our expertise in the field

Strong application knowledge, a comprehensive, scalable portfolio and compliance support helps you quickly achieve business and application success. We are the leader in Raman spectroscopy, an ideal solution for inline measurements of multiple biochemical parameters with a single probe. Our Raman technology is scalable from micro-bioreactors to cGMP manufacturing and is proven to help achieve plant efficiency savings and increased yields when the technology scales to manufacturing.

To ensure maximum yield and product quality, in cell growth processes it is essential to perform all cultivation steps under the same environmental conditions and by controlling various parameters. The most critical analytical process parameters are pH and dissolved oxygen (DO). Both parameters need to be controlled permanently. Furthermore, the actual cell growth and temperature in the process have an impact on performance and should also be monitored for an enhanced insight into your process.

Our expertise in the field

Accurate process control is only possible with reliable instrumentation in the bioreactor. Endress+Hauser offers a huge portfolio of inline process instruments proven in use. The digital Memosens technology for pH and DO simplifies inline process control and the inductive non-contact coupling provides safe measuring values even under moisture or harshest process conditions. The same sensors can be used in all scales of bioreactors to provide consistent measured values from lab to manufacturing.

• Improved product quality thanks to reliable DO values with the digital optical oxygen sensor Memosens COS81E
• Proven pH glass electrode Memosens CPS61E for hygiene applications to ensure maximum product yield
• Ensure process safety by continuous monitoring of cell density using our cell growth absorption sensor OUSBT66
• Reduce risks and costs by implementing fully automated, traceable inline RTD sensor TrustSens TM371. The first self-calibration
  temperature sensor helps eliminate non-conformities or undetected failures.

Maximizing bioprocess yield is critical to achieving competitive product pricing in cultivated meat. Daily offline analysis in the laboratory increases contamination risk, carries higher operational expenses, and does not provide 24/7 understanding of cell metabolism. Inline simultaneous monitoring of glucose, lactate, viable cell density, and amino acids with Raman spectroscopy allows for real-time understanding, automated feed strategies, and is a proven strategy to improve bioprocess yield.

Our expertise in the field

We apply our many years of industry experience and proven Raman technology toward optimizing cultivated meat production. Simultaneous inline monitoring of nutrients and metabolites provides better understanding and control of the bioprocess. Our Raman analyzers are proven to be scalable, reduce the time in process development, and optimize plant efficiency. In one example, real-time Raman-based glucose control improved titer by 85% in a mammalian cell culture.

It is vital that producers within the food industry continuously review production equipment to identify any weak links that could lead to contamination. Incorporating effective hygiene practices and equipment that conforms to hygienic design criteria is a smart decision. By doing this, foodborne illness risks will be reduced significantly. Cultured meat production is a biotechnology process using mainly Life Science know-how and equipment.

Our expertise in the field

Endress+Hauser knows both worlds and offers dedicated products that fulfill all relevant standards for Life Science with ASME BPE compliance and for the food and beverages industry. They also meet hygienic standards like 3-A as well as EHEDG (European Hygienic Engineering and Design Group), which is an international network of equipment manufacturers, food processing companies, research institutes, and public health authorities dedicated to improving hygiene food production.

• Our products comply with worldwide food contact material requirements, such as FDA, EC 1935/2004, and GB 4806.
• Take a closer look at our portfolio of process instrumentation. Process-related measuring tasks can be equipped with instrumentation according to hygienic design.
  
• Learn more about process instrumentation to optimize your Cleaning-in-Place (CIP) process.

Bioprocesses undergo several changes in scale and volume. Even just within the laboratory, there are scalability considerations when going from inoculation to expansion. As bioprocesses enter a commercial manufacturing phase, there are increased operational and compliance demands to ensure a consistent product quality. A seamless transfer of process development from the laboratory to manufacturing is critical to supporting plant efficiency and process yield goals in a regulated environment.

Our expertise in the field

Endress+Hauser supports you from the laboratory to the process with fit-for-purpose analysis products and scalability know-how. Our project and commissioning services are proven to reduce plant engineering costs and time by 30% and support the scaling process with an offering fit for pilot plants up to larger scale plants. Moreover, our comprehensive portfolio makes it possible to be a unique one-stop supplier in the field of process instrumentation.